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a) RNA-seq results of indicated genes that mark WT <t>(eGFP+,</t> Neomycin-resistant) and optoWnt <t>(Cry-LRP6c-2A-mCherry+,</t> Puromycin-resistant) cells. Graphs show mean expression (read count + 1) ± 1 s.d., n = 3 biological replicates. b) Top upregulated (red) and downregulated (grey) genes in illuminated optoWnt vs. unilluminated optoWnt hESCs. Graph shows mean log 2 fold change for each indicated gene. c) Heat map of mRNA expression (read count + 1) of indicated lineage markers. Biological replicates displayed for each condition, with undetected genes (read count < 150) shown in grey. d) qPCR validation of RNA-seq results with indicated lineage markers and comparison to CHIR (3µM) treatment. Graphs show mean fold change ± 1 s.d., n = 3 biological replicates. ANOVA followed by Tukey test. e) Heat map of mean log2 fold change in lineage markers normalized to WT expression level, from qPCR data shown in (d).
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a) RNA-seq results of indicated genes that mark WT <t>(eGFP+,</t> Neomycin-resistant) and optoWnt <t>(Cry-LRP6c-2A-mCherry+,</t> Puromycin-resistant) cells. Graphs show mean expression (read count + 1) ± 1 s.d., n = 3 biological replicates. b) Top upregulated (red) and downregulated (grey) genes in illuminated optoWnt vs. unilluminated optoWnt hESCs. Graph shows mean log 2 fold change for each indicated gene. c) Heat map of mRNA expression (read count + 1) of indicated lineage markers. Biological replicates displayed for each condition, with undetected genes (read count < 150) shown in grey. d) qPCR validation of RNA-seq results with indicated lineage markers and comparison to CHIR (3µM) treatment. Graphs show mean fold change ± 1 s.d., n = 3 biological replicates. ANOVA followed by Tukey test. e) Heat map of mean log2 fold change in lineage markers normalized to WT expression level, from qPCR data shown in (d).
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Image Search Results


KEY RESOURCES TABLE

Journal: Neuron

Article Title: Orchestrating Opiate-Associated Memories in Thalamic Circuits

doi: 10.1016/j.neuron.2020.06.028

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-c-Fos Santa Cruz Biotechnology SC-52G Donkey anti-rabbit 647 Invitrogen A-31573 Bacterial and Virus Strains AAV1.hSyn.eGFP.WPRE.bGH University of Pennsylvania vector core or Addgene Addgene: 50465-AAV1 AAV1.hSynp.hChR2(H134R)-eYFP.WPRE.hGH University of Pennsylvania vector core or Addgene Addgene: 26973-AAV1 AAV1.CAG.ArchT.GFP.WPRE.SV40 University of Pennsylvania vector core or Addgene Addgene: 29777-AAV1 AAV8.hSynp.hM4D-mCherry This manuscript N/A rAAV2-retro-CAG.nls-mRuby3 This manuscript N/A AAV9-hEF1a-DIO-hM4D-mCherry-WPRE-pA Taitool Bioscience N/A rAAV2-retro-CMV-bGI-Cre-EGFP-pA Taitool Bioscience N/A AAV9.hSyn.GCaMP6m BrainVTA Co.,Ltd.

Techniques: Plasmid Preparation, Recombinant, Software

a) RNA-seq results of indicated genes that mark WT (eGFP+, Neomycin-resistant) and optoWnt (Cry-LRP6c-2A-mCherry+, Puromycin-resistant) cells. Graphs show mean expression (read count + 1) ± 1 s.d., n = 3 biological replicates. b) Top upregulated (red) and downregulated (grey) genes in illuminated optoWnt vs. unilluminated optoWnt hESCs. Graph shows mean log 2 fold change for each indicated gene. c) Heat map of mRNA expression (read count + 1) of indicated lineage markers. Biological replicates displayed for each condition, with undetected genes (read count < 150) shown in grey. d) qPCR validation of RNA-seq results with indicated lineage markers and comparison to CHIR (3µM) treatment. Graphs show mean fold change ± 1 s.d., n = 3 biological replicates. ANOVA followed by Tukey test. e) Heat map of mean log2 fold change in lineage markers normalized to WT expression level, from qPCR data shown in (d).

Journal: bioRxiv

Article Title: Optogenetic control of Wnt signaling for modeling early embryogenic patterning with human pluripotent stem cells

doi: 10.1101/665695

Figure Lengend Snippet: a) RNA-seq results of indicated genes that mark WT (eGFP+, Neomycin-resistant) and optoWnt (Cry-LRP6c-2A-mCherry+, Puromycin-resistant) cells. Graphs show mean expression (read count + 1) ± 1 s.d., n = 3 biological replicates. b) Top upregulated (red) and downregulated (grey) genes in illuminated optoWnt vs. unilluminated optoWnt hESCs. Graph shows mean log 2 fold change for each indicated gene. c) Heat map of mRNA expression (read count + 1) of indicated lineage markers. Biological replicates displayed for each condition, with undetected genes (read count < 150) shown in grey. d) qPCR validation of RNA-seq results with indicated lineage markers and comparison to CHIR (3µM) treatment. Graphs show mean fold change ± 1 s.d., n = 3 biological replicates. ANOVA followed by Tukey test. e) Heat map of mean log2 fold change in lineage markers normalized to WT expression level, from qPCR data shown in (d).

Article Snippet: To generate the Brachyury-2A-eGFP donor plasmid (manuscript under revision), DNA fragments of ∼2 kbp in length were PCR-amplified from the endogenous genomic T locus, before and after the stop codon, and were cloned into the OCT4-2A-eGFP donor plasmid (Addgene plasmid #31938).

Techniques: RNA Sequencing Assay, Expressing